FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_84_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_84_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3091162
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG41230.13338026282672988No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG36800.1190490825133073No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT36520.11814327427679298No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGCGGGT34200.11063800603138883No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC34150.11047625456058273No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA32830.10620601573130103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGT4950.087.840166
ACGTGCG950.083.913179
TGACGCC6700.081.167015145
GACGAAA13550.075.45238145
AAACACG15400.075.301315
CACGAGC10100.071.751788
ACACGTG6400.069.0722967
GAAACAC17200.068.2636954
TAGTAAC44950.065.472042
ACACGAG11200.064.704737
ACGAGCC2600.064.108499
AACACGA11650.062.2043956
GCCGACC2800.062.15074145
TAACAAG46400.061.38725
GTAACAA46850.060.6428644
ACGAGCG851.3107638E-859.681569
ACGTGCC2000.057.9763769
ACGAGCA7800.055.7465139
CGCCGGG805.8726073E-754.3819145
AGAAGCG7350.051.2766045
Produced by FastQC (version 0.11.5)