FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_81_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_81_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5123885
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT183790.3586926716739349No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCCT154060.30067029217088204No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA129340.25242564967792996No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT96110.1875725157766031No Hit
CATAATAATTGAGGGGCTTTCTGCCGAAGAGATAATAAAAATGGGTGAAA87430.17063224486888368No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC83530.1630208328250927No Hit
TAATAATTGAGGGGCTTTCTGCCGAAGAGATAATAAAAATGGGTGAAACA81910.15985916936074873No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA77060.1503936954088548No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG75170.14670508803378687No Hit
GGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGAAAATA74090.14459731239089088No Hit
TGCTAAAGCAGAGCTCCATGGGAAATTCAGCTCTTGTGAAGAAATATCTA72830.14213824080751225No Hit
GGCTTGGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGA72010.14053789263420235No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC70820.13821543613878923No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC69730.13608814405475533No Hit
TTCAATACCTCCATGTTGTCAGAGAGTACTTCCCTCATTGTTTCTTTGGC68410.13351197382454916No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA65620.1280668867470679No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG62770.12250470102275911No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG62260.1215093625247249No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG62240.12147032964244904No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT58320.1138198847163822No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT56540.11034595819383143No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC56210.10970191563627989No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG55310.10794543593386659No Hit
CCTCCATGTTGTCAGAGAGTACTTCCCTCATTGTTTCTTTGGCTTGGATT54230.10583766029097064No Hit
GTCAGAGAGTACTTCCCTCATTGTTTCTTTGGCTTGGATTTCTTTTTGGT53280.10398359838286769No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT53250.10392504905945392No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT53150.10372988464807464No Hit
GTGCTAAAGCAGAGCTCCATGGGAAATTCAGCTCTTGTGAAGAAATATCT52030.10154404324062699No Hit
AAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATCCAAG51690.10088048424193752No Hit
GGTCAGTACAGTGGGTTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA51540.10058773762486864No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA51240.10000224439073087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA34650.082.41889
TGACGCC12250.079.90523145
GACGAAA28050.078.83961145
GTAACAA47150.071.800574
AACAACG41500.069.338037
GCTGCCC12950.068.30737145
GCCGACC6750.066.59865145
TAACAAG55950.061.0252045
ACAACGC49950.057.8983888
TAGTAAC61350.057.313082
GTCGCCC10600.056.090008145
CGCACCC1301.8189894E-1255.774303145
TACCGAT1258.913048E-1152.1869057
AGTAACA69100.050.6742323
CACGAGC21050.049.583768
CGGCCAG450.00963792548.33773145
AAACACG32450.047.1363755
GAGCAGA26550.044.789691
GACGCCC1307.354174E-944.619442145
ACACGAG24000.043.4890867
Produced by FastQC (version 0.11.5)