FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_80_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_80_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2736623
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG28260.10326595954210718No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA27450.1003061071985436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC37250.087.771352
GTAGTAA39350.084.579631
AGTAACA44600.072.327563
AACACGA26400.063.4137656
AAACACG38400.063.044375
GTAACAA52550.060.828194
GAAACAC40200.060.4028934
ACAACGC11300.060.289378
ACACGAG27850.058.813177
AACACGT13200.058.1979146
GACGAAA13550.057.25776145
CACGAGC28750.056.4678888
ACGAGCC4950.054.1736269
TAGAAAC63550.051.9037062
AACAACG13800.049.892577
ATTAGTA21800.049.892191
GTAGAAA68150.048.7301141
CAACGCA15600.047.3878449
TAACAAG71050.045.192955
AGAAGCG15950.044.9889225
Produced by FastQC (version 0.11.5)