FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_79_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_79_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3858938
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT181400.4700775187370204No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT123250.31938839131387964No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA117980.3057317842370103No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA105840.2742723516159109No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC100010.25916456807546534No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT91560.23726735179471656No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC89410.23169587072920061No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC87710.22729051360762986No Hit
TTTGTGTATCGCATGTATTCAATGATGACCAATAACCCCATAAACATCTT86430.2239735388337413No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT86380.2238439695066363No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA80940.20974682671760989No Hit
GATTAAGAATGGATGCACGATTAGATTATGAATCAGGGAGAATGTCAAAG77550.20096202633988935No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG75060.19450947385005926No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA70390.1824076986984502No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT70260.18207081844797715No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT64590.16737765675426763No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG63840.1654341168476923No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT63370.16421616517290508No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT60380.15646791941202476No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT59540.15429115471666038No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG56850.14732032491841018No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT55750.14446979972209972No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC55400.14356281443236454No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC54800.14200798250710428No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT52620.1363587598453253No Hit
CCCCATAAACATCTTCGAAGCTTATATGTACCCAATCTCACCAAGGTGAG52050.13488166951632807No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC51010.1321866275125436No Hit
ATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGT50130.1299062073554952No Hit
GTATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGT49880.1292583607199701No Hit
TTCCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTA49700.12879191114239202No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT49410.12804040904518288No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC49370.12793675358349887No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA48630.12601912754234454No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT47610.1233759132694021No Hit
ATGTTATATTGGTCTACTGTTGTTTGCTTCAAAACTGTTTTGGCCTCATT47550.12322043007687608No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG47400.122831722095561No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAACAGACGGATTCATAA47010.12182108134414184No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG46160.11961840278335645No Hit
ACACAATAGACACCGTGATCAGAACGCATGAGTACTCAAACAAGGGGAAA45050.11674196372162496No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT44910.1163791696057309No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG44340.11490207927673365No Hit
ACATAGCATGCTTGAGGCTATGGCCCACAGATTAAGAATGGATGCACGAT44240.1146429406225236No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG44150.11440971583373456No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT43620.11303628096642132No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC42530.11021166963553185No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT42510.11015984190468983No Hit
GTATGGCTCAAACCCTTCAATTCCAACAGACGGATTCATAAATAGGAGTA42250.10948608140374373No Hit
CACCAAGGTGAGCCATTGCTTTCTCAAAATCATCCTTTGACATTCTCCCT41560.10769802468969443No Hit
CATGTATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATA41020.10629867595696019No Hit
ACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA40830.10580631251396108No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT40030.10373320328028073No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG39970.1035777200877547No Hit
TATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATCATC39270.10176374950828442No Hit
TATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATCATCCT39130.10140095539239034No Hit
TTCATAATCTAATCGTGCATCCATTCTTAATCTGTGGGCCATAGCCTCAA39070.10124547219986432No Hit
GTGTATCGCATGTATTCAATGATGACCAATAACCCCATAAACATCTTCGA39070.10124547219986432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAA450.0128.90482145
GTAACAA60700.099.473464
TGACGCC8350.098.1259145
GCTGCCC5850.097.91809145
TAACAAG62450.096.6834645
GAGCAGA10450.088.116361
GAAACAC34450.087.9504244
GTCACGT259.384186E-487.01076145
TATCGGG502.2684617E-887.01076145
AAACACG35250.085.9521565
TAGTAAC71600.085.4526142
GACGAAA14300.082.65008145
GACGACC451.2326509E-680.56551145
AGTAACA76550.079.9238363
CGCACCC950.076.325226145
AACACGA30000.076.093946
AACACGT10050.074.994286
ACACGAG30650.074.483117
ACACGTG10600.071.105837
CAACGCA6100.068.909889
Produced by FastQC (version 0.11.5)