FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_78_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_78_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3048046
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA79820.26187268827307725No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA54570.1790327311333228No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA41800.13713703795808857No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC41270.13539821905574914No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT39820.13064107300217911No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG36200.11876461182016283No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC34340.11266234171006606No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAATCTCAGTAAGAACAATT33890.11118598603826846No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG30990.10167169393112835No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT30900.10137642279676883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA24050.0101.889632
TAGTAAC92550.095.959512
GTAACAA93700.094.466084
AGCACGC26150.093.993791
GTAGTAA95500.093.4600141
CACGCAC29200.085.157993
AGTAACA105850.083.899493
CGCACTT30750.081.804485
TAACAAG112050.079.123835
AAACACG40850.075.597995
ACGCACT33600.075.5142064
GACGAAA9050.074.509674145
ACGAGCG1950.070.620919
TGACGCC7250.070.006485145
GTCTCGC19100.067.950211
ACAAGAG138000.064.3374947
CTCGCTC20450.062.7471583
CAAGAGC132150.061.7010468
GAAACAC51350.060.4231224
AACAAGA148600.059.796926
Produced by FastQC (version 0.11.5)