FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_75_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_75_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036921
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCT26080.12803638432712902No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC21920.10761340277801643No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA26250.088.10511
TAGTAAC27400.081.747212
AGCACGC16950.073.141881
TAACAAG32800.067.620845
CGACGAA3700.066.63334145
GCACGCA19000.065.239032
ACGCACT19950.062.4911584
GTAACAA36600.060.7981454
CGCACTT15750.060.7472155
AAACACG24350.060.426875
AGTAACA38050.058.8636933
ACACGAG17750.052.263927
GTAGAAA35950.049.2074241
AACACGA19700.048.562176
AACACGT11450.048.1059156
CACGCAC26850.046.4331173
TAGAAAC40300.043.5286672
CAAGAGC49800.042.932198
CACGAGC21300.042.5324868
TCGCTCT18950.042.074264
Produced by FastQC (version 0.11.5)