FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_73_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_73_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4610100
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC102380.22207761219930153No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG90330.19593935055638706No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA87180.1891065269733845No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT82230.1783692327715234No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG69120.14993167176416997No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT66600.14446541289776796No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC65350.14175397496800501No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC63850.13850024945228956No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT58070.12596256046506585No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA57570.12487798529316066No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG51330.11134248714778422No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA51010.1106483590377649No Hit
AAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACA49570.10752478254267804No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT48010.10414090800633392No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA47520.10307802433786686No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG47280.10255742825535238No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC46960.10186330014533307No Hit
GGCTTGGGCTGTCCCAAAGGACAACAACAAAAATGCAACGAACCCATTAA46740.1013860870696948No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT46340.10051842693217068No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC10850.0107.579341
GCACGCA11000.0105.429232
CACGCAC11300.0103.271673
GAAACAC19950.099.545714
AAACACG20050.099.0492255
TGACGCC6300.092.07337145
ACAACGC10400.082.2282648
AACACGA19750.081.826016
ACACGAG19900.080.848587
GCTGCCC6500.080.31631145
GACGAAA14450.076.77295145
CAACGCA11500.075.624169
AACACGT5250.073.159416
ACGAGCC5750.071.842959
CACGAGC23200.067.474288
GGCACCC2950.066.36306145
TAACAAG105250.064.8699655
CGCACCC902.8194336E-1064.45136145
ACACGTG5800.063.7257087
ACGTGCA7350.060.1556242
Produced by FastQC (version 0.11.5)