FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_6_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_6_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4015411
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT61370.15283616048270027No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG59750.14880170423401243No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA59500.14817910296106676No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA59200.14743198143353198No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC58610.1459626424293802No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA56770.14138029706050015No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG54610.13600102206224968No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT52270.1301734741474783No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG50230.12509304776024174No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC49970.12444554243637825No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG49430.12310072368681563No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGCGGGT49260.12267735482121257No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA47320.11784596894315426No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG46710.11632682183716685No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT46390.1155298922077964No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT45800.11406055320364467No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT45220.11261611825041073No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC44380.11052417797331332No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC44010.10960272808935374No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC43440.10818319718703764No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT43420.10813338908520198No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC43050.10721193920124242No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA41650.10372537207274672No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT41230.10267940193419801No Hit
GTCTACACCTGTGACAGTGTCCCACAGCAGCTGTCCTGACCCCATTAAAC40370.10053765355526496No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCC256.463353E-6116.01412145
TGACGCC9050.085.728676145
AAACACG17100.081.3951645
GTAACAA54500.079.543294
ACACGAG11600.076.226997
TATCGGG1150.075.661385145
AACACGT6650.071.9331746
CACGAGC12350.070.424088
TAACAAG61900.070.03325
AACACGA13550.066.861736
ACGAGCC4150.066.366339
ACACGTG7150.065.88927
GCCGACC5950.065.806335145
TAGTAAC69150.064.265522
GCTGCCC8000.060.726143145
AGTAACA73450.060.6019063
GAAACAC23350.059.609194
GTCGCCC11950.054.609158145
GAAGCGG11250.053.4914051
AGAAGCG6250.053.354535
Produced by FastQC (version 0.11.5)