FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_68_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_68_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115213
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA72510.34280235607477827No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC51180.24196144785418772No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA50890.24059042753613938No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT35820.1693446475603166No Hit
AGTAGAAACAACGCACTTAGCCGATAAAGAATTTTTTGGCTAGAGGGCTT33870.16012571783550877No Hit
TCTCTCTTCTGTCCTTCCAAGCCTACTAAGCACGTGGCATCCTCCCAGAT33780.15970022877128687No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA33480.1582819318905472No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA33460.15818737876516453No Hit
GTAGTAACAAGAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTA30820.14570636621465546No Hit
CTGCATATCTGCTCCTTTGTATAAAGGATAAACAAAAACTTTAAAGTACT30320.14334253808008934No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG29600.13993862556631415No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG29590.1398913490036228No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC29290.13847305212288313No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC27220.12868680364577942No Hit
CCCTTAGACAGTGCCGTTCAATGTTTTCAAATCTTGTTACTATTACTCTT25260.11942059735828023No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG23640.111761794202286No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC22100.10448120354782237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA45200.073.469771
TAGTAAC50050.066.4763262
GCCGACC5600.064.74018145
TGACGCC7900.064.24848145
ACAACGC18450.062.454928
AACAACG19900.059.3608977
CAACGCA30800.055.0606549
GACGCTC2400.054.381752145
ACACGAG22200.053.2108967
AAACACG33900.052.169625
TAACAAG64700.051.3084345
AGTAACA65200.051.25213
CACGAGC23150.051.0272948
GTAACAA65700.050.749334
ACGAGCC9350.049.607189
AACACGA25150.047.8327946
CGACGAA15150.047.38212145
AAGAGCG9150.044.3550579
ACGAGCA10250.043.8373229
GTCGCCC8450.043.76283145
Produced by FastQC (version 0.11.5)