FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_67_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_67_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2409232
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA57670.23937088665599662No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA39690.1647412951513179No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA28990.12032880187545242No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT26320.10924643205801683No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC25220.1046806617212456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC63000.095.250352
GTAGTAA64400.093.307731
AGCACGC18050.091.5601651
GCACGCA18200.090.790472
AAACACG37700.083.035745
GTAACAA76300.077.8774954
AGTAACA77200.077.540843
CGCACTT22400.076.346065
TAACAAG78850.075.358945
GAAACAC44800.070.03784
AACACGT13300.069.187826
GACGAAA9550.069.10119145
CACGCAC24300.068.2962653
ACGCACT25950.067.8565144
AACACGA32700.067.581786
ACACGAG35500.062.252687
CACGAGC38250.057.5875668
ACGAGCA19150.055.620539
ACAAGAG107400.055.5241747
CAAGAGC103450.054.632458
Produced by FastQC (version 0.11.5)