FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_66_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_66_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161928
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT67040.31009358313505353No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC64450.29811353569591587No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT51240.23701066825537204No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT51130.23650186315177935No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT46120.21332810343360187No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT43920.2031520013617475No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC40680.18816537831047103No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG34090.1576833271043254No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT32000.14801603013606374No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT31360.14505570953334246No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC28220.1305316365762412No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAATCTTCTC25350.11725644887341298No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC24820.11480493337428443No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT24570.11364855813884643No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG23230.10745038687689876No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC23220.10740413186748124No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG23170.10717285682039365No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA22850.10569269651903301No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT22720.10509138139660525No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT22610.10458257629301253No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT22270.10300990597281687No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA22120.10231608083155405No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA22110.10226982582213653No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA21660.10018835039834813No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAC100.0070919343144.996541
GTAACAA46400.0120.7504044
AGCACGC7500.0116.963871
GCACGCA7850.0111.741162
CACGCAC7900.0111.026223
GAAACAC15600.0108.722224
TGACGCC2350.0107.99613145
TAACAAG52000.0107.607135
AAACACG16300.0104.053185
GAGCAGA5050.094.7502061
TAGTAAC68550.083.121342
GCGCAAA353.4354318E-582.87051145
TAGAAAC22500.082.158992
TTCGGAA1250.081.2131145
GACCCCC1300.078.089516145
AGTAACA73300.077.63063
ACGCACT11950.075.2109154
CGCACTT11900.074.917845
AACACGA19100.073.98236
GCTGCCC2950.073.74071145
Produced by FastQC (version 0.11.5)