FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_64_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_64_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3468513
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC107060.3086625306002889No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT102870.2965824259560221No Hit
TTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACAA89530.25812214052534904No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT74460.2146741269241315No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT68360.197087339733194No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC63980.18445944991412747No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT54120.15603228242189088No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC44920.12950794764211637No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA44470.1282105617018013No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA40900.11791796657530187No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC39740.11457359392915639No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC39640.11428528594241971No Hit
ATATCCGAATATAGTAAGTATGATAATGAAGCTGACACATATAGTGATAA38630.11137337527637925No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT37460.10800017183156009No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG37140.10707758627400271No Hit
ATTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACA36560.10540539995092998No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA36420.10500176876949863No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG36360.10482878397745662No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA36250.10451164519204627No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT35650.1027817972716262No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC35200.10148441133131114No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA35190.10145558053263747No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG34900.1006194873711011No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT34820.10038884098171176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA66850.0115.912564
AGCACGC5650.0111.638171
GAAACAC24500.0109.468674
GCACGCA5750.0108.423242
CACGCAC5850.0105.327633
AAACACG25500.0104.604245
TAACAAG86050.090.468025
GACGAAA10950.090.05686145
TAGAAAC32500.088.552112
TAGTAAC95700.081.809472
ACGTGCA9800.076.930592
AACACGT5950.076.739936
TGACGCC4350.076.6762145
AACACGA29800.074.422286
GAAGCGG9650.072.8763051
ACGCACT9200.071.698124
CGCACTT9050.071.282535
AGTAACA110450.070.882223
ACACGAG31950.069.189367
ACAAGAG113550.068.4893347
Produced by FastQC (version 0.11.5)