FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_63_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_63_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164165
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC37900.17512527926475108No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG35390.16352727264325964No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA28190.13025809030272645No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC28050.12961118953499387No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG203.8695917E-4108.729363
GACGAAA9900.086.42772145
AGCACGC7950.077.5243151
GCACGCA8150.077.3797452
GTAACAA49950.072.838934
TAGTAAC54750.068.4497762
TAACAAG53650.067.815555
AAACACG19000.064.084215
TGACGCC6350.061.663235145
CACGCAC10350.061.630823
GTAGTAA65600.059.2443731
AACACGA14100.059.1022226
AGTAACA64100.058.803193
ACACGAG14600.057.0794877
TCGACGG651.0930542E-555.777992145
CGCACTT10950.055.5981675
ACAACGC7050.053.45028
ACGAGCC5600.051.7615249
GAAACAC24700.050.175824
AACACGT7800.050.16776
Produced by FastQC (version 0.11.5)