FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_61_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_61_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815800
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT37250.2051437382971693No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC29120.16037008481110254No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG23570.12980504460843703No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC20180.11113558761978193No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC10250.096.912461
GCACGCA10600.094.3731462
GACGAAA6850.083.626396145
CACGCAC12150.082.333773
GTAACAA44550.078.419974
TGACGCC3100.074.85058145
AAACACG15800.074.318555
CGCACTT13350.072.212045
TAGTAAC48250.071.9636842
GAAACAC17100.068.666714
TAACAAG52000.067.047265
AACACGA12200.066.531256
ACGCACT15700.066.01824
ACGAGCG554.0724153E-665.883229
GTAGTAA54650.064.8786241
ACACGAG12400.064.873727
ACAACGC6300.064.419158
AGTAACA54800.063.759023
GAAGCGG4650.060.8127371
AACACGT5950.059.682446
Produced by FastQC (version 0.11.5)