FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_5_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_5_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730083
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA25100.1450797447290101No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT24920.14403933221700924No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA24680.14265211553434143No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG23460.13560043073077996No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG22940.13259479458499968No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC22030.12733493132988416No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG21550.12456049796454852No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGCGGGT21370.12352008545254765No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC20590.11901163123387722No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA19850.11473437979565142No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT19570.11311596033253896No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT19060.11016812488186982No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG18880.10912771236986896No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT18740.10831850263831272No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA18010.1040990518951981No Hit
GTCTACACCTGTGACAGTGTCCCACAGCAGCTGTCCTGACCCCATTAAAC17950.10375224772453114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGT2900.084.968566
TAGTAAC24250.075.028672
ATCGAAA300.001935047872.50882145
GAAACAC9000.072.485764
GCCGACC4450.071.69412145
AAACACG9100.071.687135
GTAACAA26850.066.68154
ACACGAG6200.066.628577
AACACGA6300.065.570986
TGACGCC4450.065.176476145
TATCGGG350.00356522462.15042145
TAACAAG29250.061.208435
CAACGCA4900.060.642589
CACGAGC6700.060.5746048
CTTACGG851.3047611E-859.713146145
GAAGCGG5700.058.510811
ACACGTG4300.057.3043757
GTCGCCC5450.057.208794145
GAGTGCG400.006060492254.354897
ACGTGTT7500.053.173134145
Produced by FastQC (version 0.11.5)