FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_57_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_57_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3660110
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC68810.18799981421323403No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT49160.13431290316411254No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA46810.12789233110480286No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG46180.12617107136124325No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC42640.11649923089743204No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA42610.11641726614773874No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT39580.1081388264287139No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC37750.10313897669742166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA72450.088.751424
GACGAAA12250.087.01062145
TAACAAG78650.081.659615
AAACACG27650.081.5337755
ACGAGCG451.2359596E-680.529069
AGCACGC11850.079.545581
TGACGCC7500.078.30956145
GCACGCA12450.076.282982
GAAACAC30200.074.412324
TAGTAAC91400.071.387482
GCCGACC3050.071.32018145
ACACGAG24900.071.3109057
CACGCAC14000.068.354273
AACACGA26550.067.971066
ACGCACT14550.067.758854
CGCACTT13300.067.5837555
AGTAACA101700.064.227883
CACGAGC28800.061.1509178
AACACGT8350.057.285756
ACGAGCC8950.056.685269
Produced by FastQC (version 0.11.5)