FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_56_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_56_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3198505
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC56370.17623858646461393No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA51610.16135663380235454No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT49490.15472853723849112No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA82250.093.5818254
TAGTAAC87700.088.021122
GCACGCA17300.086.7283552
AGCACGC17500.086.583171
TAACAAG89900.085.859045
CGCACTT18700.082.940825
AAACACG37150.082.52355
GACGAAA11450.078.53017145
AGTAACA100200.077.3261643
ACGCACT21050.075.7484054
CACGCAC20500.073.186843
GTCTCGC11500.068.084951
GAAACAC46600.065.789624
GTAGTAA119050.065.281561
AACACGA35500.063.2822466
ACAAGAG125300.061.308937
TGACGCC5650.060.32117145
GCCGACC2550.059.717205145
CAAGAGC111750.059.2096068
ACACGAG38350.058.5821467
Produced by FastQC (version 0.11.5)