FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_55_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_55_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261836
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAAGGGACATCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTT26620.21096243885893254No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT26590.210724690054809No Hit
GTTTAGGCGGTCTTGACCGGGGTAGTCAAGGGCTCTTTGCCATGAAAGCC23100.18306657917510674No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC22930.18171933595174017No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG22630.179341847910505No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA21130.16745440770432926No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG20440.1619861852094884No Hit
AGTAGAAACAACGCACTTGGCCTCGATCACGTGAAAGATCCGAATGCGAT18690.14811750496895001No Hit
GTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACAACAACATGACCACA17940.14217378486586213No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT17480.1385283032026349No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC17050.13512057034353117No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA17040.13504132074215666No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA17020.13488282153940764No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA16640.1318713366871765No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAG16150.1279881062198257No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG15960.1264823637937101No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT15470.12259913332635937No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG15300.12125189010299278No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA15250.12085564209612025No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC14550.1153081699999049No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT14540.1152289203985304No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC14520.1150704211957814No Hit
GGGTAGTCAAGGGCTCTTTGCCATGAAAGCCTTTCATAGCACTCCAGAAT14140.11205893634355019No Hit
CTTTTGTTGTGAGTCTTTATTCTTGACTCTAATTTTCTTTTTAGTCTGTT13900.11015694591056206No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA13810.10944369949819152No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG13620.10793795707207594No Hit
GATTAAGAATGGATGCACGATTAGATTATGAATCAGGGAGAATGTCAAAG13610.10785870747070142No Hit
TTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTATGAAC13350.10579821783496428No Hit
TCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTTATTCTTGACTCT13230.10484722261847022No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAA13090.10373772819922716No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT12810.10151873936074102No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA100.007088761145.01587145
TGACGCC3900.083.662994145
GCCGACC2750.076.462906145
ACACGAG5450.071.811397
GTAACAA12400.071.3129964
AACACGA5500.071.155756
CAACGCA7250.068.977469
TAACAAG13250.066.7382055
CGACGAA8050.066.65325145
AAACACG8450.066.0487445
CACGAGC5850.065.662318
ACGAGCC3350.064.904179
ATAACTA350.003565174562.149654145
AACTCGA350.003565174562.149654145
TCTGACC2650.060.195263145
TAGTAAC15150.058.3799632
ACAACGC8400.055.220058
GCTGCCC4600.055.16908145
TCTCGGG10300.053.501145
AGTAACA16650.053.114193
Produced by FastQC (version 0.11.5)