FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_53_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_53_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272260
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA17690.1390439061198183No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA14050.1104334019775832No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG13220.10390957823086476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC22650.082.250932
GTAGTAA24400.077.546241
CGACGAA4700.074.05818145
AACACGA9950.064.8246846
AGTAACA28800.064.686933
ACACGAG10050.064.179667
GTAACAA29250.062.9408384
AAACACG14850.062.4752775
TAACAAG30250.060.380935
CACGAGC10350.060.2187278
ACGAGCC3900.057.606379
CAACGCA7300.048.6459479
AGCACGC4050.048.3302231
ACGAGCG450.009654175548.315029
CGGAGCG450.009654175548.315029
AACAACG6700.046.5122227
GTAGAAA32450.044.457851
GCACGCA4250.044.34662
CAAGAGC37500.043.290268
TAGAAAC32400.043.1805532
Produced by FastQC (version 0.11.5)