FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_51_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_51_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4027919
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG103180.2561620529111931No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA99050.24590861931434072No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA92300.22915058619599848No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG80580.20005367535941015No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC76250.18930370744794026No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA75340.1870444763164304No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG72490.17996886233313034No Hit
CATATGACCAGAGTGGAAGACTTGTTGCTAAACTTGTTGCTACTGATGAT67990.1687968402542355No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAATTCACTC66590.16532110005191267No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT66430.16492387260021862No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA65580.16281360176309403No Hit
CTTCAAGAGACATCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTT65180.16182053313385897No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC60720.1507478179178876No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC60560.1503505904661936No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT60440.15005266987742305No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG53630.13314567646469555No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG52210.12962028283091095No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT51840.1287016943488685No Hit
ATATGTTATCATCAATCCTTTTACTTAAAAGATAAAGTTCTTCCGTGACT50960.12651694336455127No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT49640.12323981688807546No Hit
AGATAAAGGAGGAAGTAAACACTCAGAAAGAAGGGAAGTTCCGGTTGACA48990.12162608036556842No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTCTTCCACTCTGG48620.12070749188352596No Hit
GTTTAGTCACTGGCAAACAGGAAAAATGGCGGACAACATGACCACAACAC47370.11760415241716628No Hit
GTAAAAGGATGTCTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG46170.114624946529461No Hit
ATTATGGAGCTGTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATC45990.11417806564630521No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC45200.11221675510356588No Hit
GTTTAGGCGGTCTTGACCAGGATAGTCAAGGGCCCTTTGCCATGAAAGCC45200.11221675510356588No Hit
TTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTATGAAA44880.11142230020017779No Hit
GGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGTCAAGAATAAA44170.10965960338328552No Hit
CCTTTATCTTTTGCCTTGCATCTTTGTTGTTCATGTCCCTTAATACTATT44120.10953546980463112No Hit
GTTATCATCAATCCTTTTACTTAAAAGATAAAGTTCTTCCGTGACTAGTC43740.1085920546068578No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCATCTGGTTCTTCTTCTA43620.10829413401808727No Hit
TTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGGGAGTCCTATCCCAAT42480.10546388842476724No Hit
AATTGGGATAGGACTCCCACCGCAGTTTCAGCTGCTCGAATTGGCTTTGA41560.10317983057752651No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT41160.1021867619482914No Hit
CAATTGGACCGATTACCCTTCAACACCAGGGAGGTGCCTTGATGACATAG41020.10183918792805913No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA40880.10149161390782685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAAA24500.082.86717145
TGACGCC7900.079.85143145
ACAACGC13050.072.197778
CAACGCA13600.071.945229
GCCGACC3650.067.54242145
AGCACGC4400.062.6204341
GCACGCA3950.062.4002042
AGACGCC1400.062.15038145
GTATGCG350.00356557762.15038145
GCTGCCC10550.058.41939145
TCGCCCC753.7609425E-758.007015145
TCTGACC9050.054.48173145
GAAGCGG8000.052.5682071
ACGTGCA3050.052.279329
TAGTAAC84200.051.0560042
AACAACG18550.050.791427
GTAACAA83950.050.425224
AGCTCGC752.5570856E-548.3169678
AGTAACA91750.046.773913
TAACAAG93100.045.4682275
Produced by FastQC (version 0.11.5)