FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_50_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_50_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194147
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA80100.3650621403215008No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC58910.2684870247982473No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT45990.20960309404976057No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC45350.20668624299101201No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT37820.17236766725292335No Hit
CTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACA37320.17008887736327602No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA36190.16493881221267306No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA35840.16334365928991995No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA35830.16329808349212702No Hit
CCCATACACCGGCGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA35210.16047238402896433No Hit
CCCATACAGGCAGCAATTTCAACAACATTCCCATACACCGGCGTTCCCCC31200.1421964891139928No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA30970.14114824576475504No Hit
GATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACCGGCGT30250.13786678832366292No Hit
GATATAACCCCAGCACATGGTCCCGTGAAGAAAATGGATTATGATGCAGT29400.13399284551126248No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC28070.12793126440480057No Hit
TTCCCATACACCGGCGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCA27790.1266551420665981No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT27700.1262449598864616No Hit
GTAGAAACACGAGCCTTTTTCATTTTAATCATTTGTTTGTCACATGTATC27230.12410289739019308No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC27200.12396616999681424No Hit
ATCTGGGACTCATAGCTGGAGAACTAAAAGGAACAGATCTATACTAAATA26510.12082143994910095No Hit
ATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATA25210.11489658623601792No Hit
GATTAAGAATGGATGCACGACTAGATTATGAATCAGGAAGAATGTCAAAG23520.10719427640900998No Hit
GTAGTAACAAGAGCCTTTTTCATTTTAATCATTTGTTTGTCACATGTATC22350.10186190806723523No Hit
GTCCCAGATACTGCATCATAATCCATTTTCTTCACGGGACCATGTGCTGG21980.10017560354889622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG16350.078.0287255
ACACGAG11650.075.27317
GAAACAC17550.072.693424
GCCGACC5800.071.25826145
AACACGA13000.067.4562766
TAACAAG25650.066.9762045
CACGAGC13300.065.934718
TAGTAAC26050.065.1159742
AACACGT6450.062.9228946
ACAACGC6550.060.8557668
ACGAGCC8800.060.120229
TGACGCC7900.058.74099145
ACACGTG7650.053.052637
CGGAGCG2650.051.971755
CAACGCA7300.051.625099
GGAGCGA1152.4938345E-950.4164776
GTAACAA33700.050.1170584
GTCGCCC8050.048.639183145
GTTCGAA450.00963624448.338936145
AGTAACA35300.047.8465583
Produced by FastQC (version 0.11.5)