FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_4_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_4_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3413109
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA85980.2519110875158104No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG76850.22516128257257534No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA71610.20980871106079532No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT68320.20016940566504027No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG59990.17576350476940525No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA56070.16427837493616523No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG53460.15663138798087023No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT52740.15452187433803022No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC52470.1537308067219652No Hit
CATATGACCAGAGTGGAAAACTTGTTGCTAAACTTGTTGCTACTGATGAT52090.15261745229935522No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG52000.15235376309400023No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT50820.14889650462379023No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG50730.14863281541843523No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA50460.14784174780237022No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAATTCACTC50050.1466404969779752No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC49830.1459959233648852No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC49390.14470677613870522No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC48840.1430953421059802No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT47590.1394329920316052No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGGAAATTAGAGT47260.1384661316119702No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA47010.1377336615970952No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA46380.1358878371596102No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT42750.12525237254362517No Hit
ATATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACT42440.12434410972518017No Hit
GGTCAAGACCGCCTAAACAGACTAAAAAGGAAATTAGAGTCAAGAATAAA39320.11520288393954017No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTTTTCCACTCTGG38900.11397233431455016No Hit
GTATTGTCCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAA38710.11341565710324517No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT37370.10948961782351516No Hit
ATTATGGAGCTGTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATC36960.10828836699912016No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG36670.10743870178186515No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC36160.10594446295152016No Hit
CTTCAAGAGACATCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTT34750.10181333206762515No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAACCCTGGAAATTATTCAAT34740.10178403326703016No Hit
AGATAAAGGAGGAAGTAAACACTCAGAAAGAAGGGAAGTTCCGGTTGACA34390.10075857524620516No Hit
GCGTAATGTATTGTCCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAAC34360.10067067884442014No Hit
GGATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATGACCAGAG34360.10067067884442014No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT34200.10020189803490015No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA34160.10008470283252013No Hit
GTAAGAAGTACAATAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA34160.10008470283252013No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC9600.095.914961
CACGCAC10300.090.794973
GCACGCA10150.090.709622
TGACGCC6750.083.78697145
GCTGCCC5850.083.043304145
GCCGACC4350.080.00878145
CAACGCA11050.075.429819
ACAACGC11050.075.42768
GAAACAC9850.073.594874
TCTCGGG9700.073.25547145
GTAACAA49950.073.144034
CGCACCC702.8121576E-972.507965145
ACACGTG2200.069.181717
GGCACCC1300.066.93043145
AAACACG11150.065.011455
CGTACAC451.1936209E-464.45152145
ACACGAG9100.062.9187977
TAACAAG60750.060.3766445
CACGAGC9400.058.5976878
AACACGT2600.058.5375186
Produced by FastQC (version 0.11.5)