FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_48_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_48_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122562
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC110500.3538760799625436No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT110120.35265913054728776No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT88590.2837093386776628No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA69410.22228541819185654No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG63560.20355080219383956No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC59810.19154143296434145No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA54210.17360744158162433No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT52430.16790699432068923No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG35820.11471349488016572No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT35300.11304819568034197No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA32520.10414524995820738No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGT31400.10055845168166397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC20600.0117.547531
GCACGCA20750.0116.677222
CACGCAC22750.0107.372313
AAACACG22250.096.752735
GTAACAA77300.094.4249654
GAAACAC22700.093.87684
CGCACTT28550.087.335175
ACGCACT29400.085.54984
TAACAAG87000.083.897125
GACGAAA9850.083.1852145
GAAGCGG7700.079.0902251
TGACGCC3750.077.34506145
TAGTAAC98100.075.146652
AGTAACA106600.069.288443
AACACGA25200.068.735096
ACAACGC9750.066.1555948
GCCGACC2000.065.259895145
ACGTGCA8950.064.792492
ACACGAG26800.064.090657
AACACGT6850.062.422716
Produced by FastQC (version 0.11.5)