FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_47_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_47_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3676210
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA67970.18489150510988217No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA65170.17727496524953687No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG65110.1771117536811009No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT61160.1663669920923995No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC59130.16084500069364918No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT56480.15363648975439378No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT50470.13728813098272405No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC49340.13421431311051327No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG48950.13315343791567946No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT47760.12991640847503272No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC47490.12918195641707084No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT47000.1278490619415104No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT44090.11993330087236584No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT43760.119035637245968No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT43740.11898123338982267No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC42550.11574420394917591No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT41620.11321442463841838No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC40610.11046702990307952No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG40470.11008620291006227No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA40410.1099229913416263No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG39920.10859009686606587No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC37830.10290489389887954No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC37770.10274168233044359No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA37720.10260567269008028No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA37010.1006743357969213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA50300.097.26194
GAGCAGA5650.093.67491
GACGAAA15850.088.75487145
GCTGCCC5300.087.56365145
GAAACAC13850.085.822484
TACGGGG353.435294E-582.87274145
TGACGCC5250.080.11031145
AAACACG15750.075.009125
GCCGACC2950.073.74269145
ACACGAG12500.073.631777
GTGCTCC809.822543E-1172.51365145
TCTGACC4650.071.73393145
TAACAAG69850.070.6622855
ACACGTG3800.070.565057
TAGTAAC72550.069.4387052
TAGTAGA3950.067.896453
AACACGA13650.067.428366
GGATCCC1350.064.45658145
CACGAGC13650.063.18098
AACACGT4300.062.359816
Produced by FastQC (version 0.11.5)