FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_46_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_46_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145514
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA41680.1942658029730871No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA22680.10570893501510593No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA21870.10193361590742359No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCG100.007099302144.946179
TAGTAAC37150.075.9064942
AAACACG25500.069.069025
ACGAGCG955.456968E-1268.6587149
TGACGCC5550.067.93914145
AACACGT8650.067.864976
GTAGTAA43300.067.3095861
GCCGACC3950.064.25111145
AACACGA18050.064.2420656
CACGAGC17850.063.743838
ACGAGCA7700.063.0609979
ACACGAG18700.061.233947
GAAACAC29200.061.0632064
AGTAACA48250.058.5929373
TAACAAG48400.057.8041735
CGGAGCG1800.056.373215
GTAACAA52850.053.212554
TAGAAAC41900.052.4223822
ACGAGCC8100.051.8943069
CAACGCA11600.051.2309729
Produced by FastQC (version 0.11.5)