FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_42_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_42_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684029
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT77850.21131755477494882No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC72530.19687684326046292No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT68510.1859648770408702No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT63720.17296280783891765No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC63000.17100842582943837No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA46450.12608478380599067No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC42830.11625858536944199No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT42440.11519996178097404No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT42430.11517281758639793No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA42400.11509138500266963No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC41720.11324557977149474No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT41080.11150835131862426No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG40290.10936395994711225No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC39750.10789817344000278No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT39630.10757244310508957No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC39620.10754529891051345No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA39370.10686669404611093No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC38890.10556377270645807No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG38600.10477659106375113No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT38190.10366367908613097No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA37930.10295793002715234No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG37840.10271363227596743No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA37770.10252362291393471No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA37640.1021707483844454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA59950.0107.969474
AGCACGC5650.098.805531
AAACACG19450.098.379665
GAAACAC19700.097.871024
GCACGCA5850.095.41722
CACGCAC6000.093.029243
GACGAAA13600.092.76513145
TAACAAG72800.089.0076145
AACACGT5550.088.7940446
GCTGCCC3950.086.273056145
TGACGCC4050.084.14285145
GCCGACC2400.081.569336145
TCCGACC451.2329328E-680.5623145
GAAGCGG8550.075.4681
CAACGCA5700.075.017539
ACACGTG6600.074.670767
TAGTAAC87900.074.4715962
ATGCGGG300.001935512572.50608145
TTCGGAA1200.072.50608145
AACACGA19650.071.9183966
Produced by FastQC (version 0.11.5)