FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_40_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_40_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993920
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC52540.26350104317124057No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT52370.26264845129192743No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT45990.23065117958594125No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC44380.22257663296421118No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT40610.2036691542288557No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA30810.1545197400096293No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC29610.14850144439094848No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT28120.1410287273310865No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA27920.14002567806130636No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC27310.13696637778847698No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC27230.1365651580805649No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG25950.13014564275397209No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT25160.12618359813834054No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG24980.12528085379553844No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC22990.11530051356122614No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA22790.114297464291446No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA22290.11178984111699566No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC21980.11023511474883647No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT21730.1089813031616113No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT21510.10787794896485316No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA21410.1073764243299631No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC21360.10712566201251807No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG20970.10516971593644679No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT20480.10271224522548547No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG20370.10216056812710639No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA20110.10085660407639224No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT20030.10045538436848017No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA41050.0111.951134
TGACGCC2150.097.80824145
AAACACG11050.093.807395
TAACAAG49400.093.324025
TTCGGAA1400.093.231145
AGCACGC3150.092.07771
GAAACAC11450.089.8949364
GACGAAA7200.085.60563145
CGGTGCA608.2945917E-1084.581522
CACGCAC3450.084.051953
GCCGACC1650.083.499825145
GCACGCA3500.082.855362
TAGTAAC62750.075.4445952
AACACGA11400.073.744446
ACAAGAG65150.070.861787
CGGAGCA11400.070.59062
ACACGAG12000.070.058977
AGTAACA69000.068.187143
AACACGT3100.067.79736
ACGTGCA5150.067.5713652
Produced by FastQC (version 0.11.5)