FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_39_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_39_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716500
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCTAAAAACAATTTTAGGTAGAAAACAGAGTGTGAGTT32750.19079522283716865No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG25790.1502475968540635No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC19500.1136032624526653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA27750.077.074711
TAGTAAC28250.075.188492
ACACGAG14600.067.514917
CGGAGCG1700.063.957865
GCCGACC5050.063.17416145
AGTAACA34100.062.714773
AACACGA16250.061.55166
TGACGCC5750.060.52734145
CACGAGC16050.059.6090938
CCGACGG1102.5465852E-1159.323666145
AAACACG25300.058.733375
ACGAGCA6750.055.8358579
AACACGT8900.052.9342776
ACAACGC9600.052.8494458
ACGAGCT5450.050.5359849
CAACGCA17350.050.129739
ACGAGCC5800.046.2367559
TAACAAG47250.045.5623635
AACAACG11500.045.3783077
GTAACAA46650.045.3727844
Produced by FastQC (version 0.11.5)