FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_38_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_38_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3744368
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT75450.20150263008336788No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC69100.18454382688881008No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT58830.15711596723398982No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT58060.1550595454292954No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC51780.138287689671528No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA47900.12792546031800292No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA46490.12415980480551057No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG41500.11083312324002342No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC39400.1052247001363114No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC39250.10482409848604625No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC39020.10420984262230636No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA38930.10396948163214727No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT38140.10185964627408416No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA37840.10105844297355387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA53200.0108.45664
AGCACGC7650.0101.41691
GCACGCA7800.0100.378732
GAAACAC17800.098.141244
TGCGAGG301.5986961E-596.68297145
AAACACG18050.096.380365
CACGCAC8200.095.4809343
GAAGCGG8350.092.0465241
TAACAAG66350.086.961425
ACGTGCA8350.077.270922
GCCGACC5600.076.39681145
AACACGT4700.074.013486
TGACGCC7750.072.98004145
CGGCGAC300.001934863772.51223145
TAGTAAC82400.072.231812
AACACGA21050.067.479436
TACGCGG2600.066.934364145
AAGCGGA11650.065.96182
TTCGGAA1150.063.054108145
AGTAACA96850.061.6785743
Produced by FastQC (version 0.11.5)