FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_37_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_37_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550562
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCGGGGGTTGGAGGAGTTGAGGGTGAAAGTCCGGGCAA21870.14104563377665646No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA18500.086.6131
AAACACG24400.071.88665
ACACGAG18300.068.513387
TAGTAAC23200.068.434742
CGCACTT5450.066.4960565
CACGAGC19150.065.850778
TAACAAG24450.064.624925
AACACGA20300.062.1142926
GCCGACC2100.055.243004145
ACGAGCA11050.053.125519
AAGAGCG8100.052.789489
ACGCACT8850.051.5981034
ACGAGCC7750.051.432949
GTAGAAA40500.049.7682461
GTAACAA32300.048.920454
GCACGCA9500.047.3138852
GAAACAC38600.046.3816344
AGTAACA34300.045.8625373
CGGAGCG1602.0008883E-1145.3004385
ACAAGAG35400.045.2448777
Produced by FastQC (version 0.11.5)