FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_35_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_35_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542155
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA31850.078.065931
TAGTAAC36750.066.0687262
TGACGCC7150.064.908966145
AACACGA18500.064.2384646
AAACACG32400.064.2002645
ACACGAG18700.063.1651577
GCCGACC6900.055.700302145
AACACGT18350.048.572686
CACGAGC24000.048.3103648
TAACAAG50750.048.2703675
GTAACAA50400.047.886564
AGTAACA52500.046.1100543
ACACGTG19800.045.382467
ACGAGCC10700.045.375629
ACAACGC11350.044.692418
GTAGAAA64000.043.720411
GAAGCGG12650.042.9781381
GCACGCA6100.042.7741282
CAACGCA11250.042.513129
CAAGAGC56450.041.7206468
Produced by FastQC (version 0.11.5)