FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_32_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_32_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3053531
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT89100.29179333695973614No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT75530.24735298249796708No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA65640.21496424958515242No Hit
CTCTACAGCAGAGGGGCCCAGCATTTTCTTCAGCTTTCTTTCAAGCGCCA63010.20635127005424214No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA61010.19980147573415824No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC60570.19836052098373982No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA59130.19364466907327943No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC52180.170884133810988No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA51170.16757648767934566No Hit
GCTCTGAGATCATCCACTTTCTCATCTAGTTCTAGTATTTCGTTGTGGAG50940.166823261332536No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA49870.16331912137129112No Hit
GTTATAATTTTACCTATTTCCCAACTTTTTGTTATTGGATCTCTCCACCA48790.15978223243844586No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA48680.15942199375084126No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC48440.15863601843243116No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA47440.15536112127238924No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA46270.15152949159514018No Hit
GAGTAGCGGTGGCAGCTGACCTTAAGAGCACTCAAGAGGCCATAAACAAG46080.1509072611347322No Hit
GCCCAAGCCATTGTTGCGAAGAATCCGTTTCCATTGGTAACGTTAGGGCA45510.1490405697535083No Hit
CCCATACACAATTGAATCTATCATTATTAACTTTCAAATTTTTGAGCCTG43960.14396447915544333No Hit
CTTCTATTGTTAATGGATTTGTTGCTGTTTTGTTTTTGTCGTTTTTTGGG43170.1413773103990102No Hit
ATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATATTCAGTGAATCAA40810.13364855310131124No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC40110.13135612508928188No Hit
TTCTCATCTAGTTCTAGTATTTCGTTGTGGAGTTCATCCATGGCACCGCT39460.12922744193525462No Hit
GCTATCATCAGTGTTACAGCCAAACTGGAGGCAGCAGTTGAGTAGTAAAG37820.12385661059278587No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC37670.12336537601877956No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA36970.12107294800675023No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG36560.11973024017113304No Hit
CTCTTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTA36420.11927175456872714No Hit
GTTTATGGCCTCTTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACTCCAT34320.1123944705326391No Hit
GGCCAGATGTTACCAGCAGCTGAACAGCATCTACAGAAACTAGCTGCAAA34050.11151024829942778No Hit
CTCAAAGAGTTGAGATTTTTTGTTATCTTGTTTATGGCCTCTTGAGTGCT32630.10685989433216823No Hit
GATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTTCCAATGAAGG32540.10656515358776447No Hit
GCCCAAAGGCCAGATGTTACCAGCAGCTGAACAGCATCTACAGAAACTAG32510.10646690667296321No Hit
ATATAGACCTCCTGCAAAACTATTAAAGGAAAGGGGTTTCTTCGGAGCTA32420.10617216592855942No Hit
AATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAAGGTACCAGCAGCT32400.10610666798535857No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA32240.10558268443975188No Hit
TACTAGAACTAGATGAGAAAGTGGATGATCTCAGAGCTGATACAATAAGC30850.1010305773872936No Hit
GGATTACGGGTAGCCCATACACAATTGAATCTATCATTATTAACTTTCAA30550.10004810823928101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGT10000.075.368196
ACACGAG11550.069.0207447
TGACGCC9450.068.293015145
GTAACAA40650.067.219064
AACACGA12300.064.810046
ATTAGTA12600.063.288721
CACGAGC12550.061.7886928
TAGGGTA3300.061.4972575
GCCGACC7100.060.25759145
TTACGCC9650.060.11471145
TACGCGG4900.059.194588145
ACGTGCC3950.058.711329
TAACAAG46650.058.4181565
GGTTAGT502.011675E-457.9954831
AAACACG27000.057.4462475
GTACCGC400.006047657754.38503145
ACGATCC14250.051.90431145
GAAGCGG11200.051.781681
CAACGCA5600.051.765569
ACGTGTC2550.051.1565559
Produced by FastQC (version 0.11.5)