FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_31_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_31_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2258371
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT39930.17680885912899164No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC39040.17286796544943236No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA34980.15489040551795963No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT26230.11614566428633735No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC24470.10835243633574822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA56700.0102.5227054
AGCACGC10000.097.144941
GCACGCA10250.094.762942
TAGTAAC64750.090.678192
TAACAAG65050.089.1397555
TGACGCC5500.085.70015145
AGTAACA74950.078.430983
AAACACG25900.075.845
CGCACTT13200.075.776395
CTCGCTC9800.075.441873
CACGCAC13050.074.427373
ACGCACT14500.069.98244
GTCTCGC10700.069.786081
GAAACAC28150.069.520694
TACGCGG1900.068.6989145
ACAAGAG85950.067.3708347
GTAGTAA89300.066.732251
ACGTGCA10050.063.470842
CAAGAGC74800.062.977128
GCCGACC2750.060.649334145
Produced by FastQC (version 0.11.5)