FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_28_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_28_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706166
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT50520.29610248944123846No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC43340.2540198315990355No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT35330.20707246539902915No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT32720.19177500899677993No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT26240.15379511723947142No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC25880.15168512325295427No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT25040.14676180395108096No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG24240.14207292842548733No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT22940.13445350569639766No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT21840.1280063018487064No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA20700.12132465422473546No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA20230.1185699398534492No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT20230.1185699398534492No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC19780.11593244737030277No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT18260.10702358387167485No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT18100.10608580876655613No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA17790.1042688695003886No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC17770.10415164761224875No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC17710.10379998194782923No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC17530.10274498495457066No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG17210.1008694347443332No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGTA100.0070886184145.01833145
GAGCCCC500.0130.51651145
GTAACAA31900.0119.308774
AGCACGC4350.0116.681421
GCACGCA4550.0111.53622
AAACACG12650.0110.6006855
GAAACAC12700.0109.597664
CACGCAC4750.0106.836813
GAGCAGA2950.0103.233391
TAACAAG39100.097.5214165
TTCGGAA607.2759576E-1296.67889145
GCCACCC301.598781E-596.67889145
GCTGCCC2300.091.42461145
TGACGCC2150.087.6855145
AACACGT3400.087.396516
AACACGA12900.085.397096
TAGTAAC45200.085.330142
GCCGACC2050.084.88878145
GGTACCC353.435819E-582.867615145
ACACGAG13400.081.672167
Produced by FastQC (version 0.11.5)