FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_24_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_24_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194788
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA33470.15249764441941543No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA29300.13349808728679033No Hit
GTAGTAACAAGAGCACAAGGGGCAAAGATGACGACAGTTTTGGCTACTTC27900.1271193390887867No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC24450.11140028102942061No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA39350.085.4833761
TAGTAAC39900.083.566842
AAACACG29350.072.600365
GCCGACC4850.070.27378145
ACACGAG20500.068.9351357
AACACGA21000.067.2907646
TGACGCC7600.065.8374145
CACGAGC22300.062.7209138
ACGAGCT4950.060.0258879
AGTAACA55650.059.653983
ACACGTG12950.058.7596867
ACAACGC17250.057.1359868
GTAACAA59700.054.510664
AACAACG19000.053.7805757
ACGAGCC9700.053.045259
ACGAGCA7550.052.7929159
ACGAGCG3750.052.1785969
CTTACGG2100.051.797543145
CAACGCA24650.051.4494869
TAACAAG63700.051.0865335
Produced by FastQC (version 0.11.5)