FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_23_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_23_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137519
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA38870.18184633680449158No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA30800.14409228643113817No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA21920.10254879605748535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC45650.087.479552
GTAGTAA46350.086.332951
AAACACG21850.077.9474645
AGTAACA58150.068.674833
GTAACAA59900.066.303934
TGACGCC5950.065.81836145
AACACGT10400.065.495126
ACACGAG16350.065.1529857
TAACAAG63800.062.9324465
AACACGA17250.061.750816
CACGAGC16700.060.7500048
ACAACGC10900.059.1695488
CAACGCA11500.057.974229
AACAACG11850.053.814477
CAAGAGC71050.050.894548
ACGAGCG901.3283534E-648.3118489
ACAAGAG83350.047.644167
ACGAGCC5650.047.456779
ACGAGCA9800.047.3258939
GAAGCGG19000.045.784121
Produced by FastQC (version 0.11.5)