FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_22_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_22_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077284
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA42920.20661594659179966No Hit
GTACTGACCAGCCATCTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG38860.18707119488716997No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA35230.16959645383106017No Hit
GGTCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA34420.16569713144663897No Hit
AGTAAGATGTTTGCCTTTTCCCCTGGTTTCAGCTTTTCAAGTTCTTCAAT32900.15837988450303378No Hit
GTATTAGCAGGCTTTCTCGTTAGTGGCAAATATGACCCAGATCTTGGAGA30470.14668191734977018No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA25940.1248745958665257No Hit
ATTTATATTAGCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGT25760.12400807978109878No Hit
GTTCCAATGGGATGCATTTGAAGCATTTGAGAGCATAATTCCTCAGAAGA25650.12347854217333788No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG25630.12338226260829044No Hit
GGTTTCAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG25270.12164923043743658No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA24550.11818316609572885No Hit
GTCCTAACTATATGCGGCAGAATGATGTCATTAAAAGGGAAAATTGAAGA23560.1134173276258807No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT23050.11096219871717108No Hit
CCAAAGAACTGGTGCAAAACACTTATCAATGGGTGCTAAAAAACTTGGTG22670.10913288698126977No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT22650.10903660741622234No Hit
AAGCAGAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGA22190.1068221774201313No Hit
CATATGATACACCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTG21990.10585938176965691No Hit
ATATGATACACCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGC21740.10465588720656395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC26750.085.634532
TGACGCC7250.079.01331145
GTAGTAA31000.075.074381
AAACACG25000.074.796565
ACGAGCG1850.070.511659
GTAACAA32750.070.156974
GCCGACC4700.069.42659145
AGTAACA33550.068.708393
AACACGA18650.067.995086
ACACGAG19150.066.2213447
AACACGT9450.064.411846
TAGAAAC38350.063.7015722
GAAACAC29700.063.695234
CGAAGCG350.003570792562.1263854
TAACAAG37850.061.8498425
ACGAGCC5450.061.1676039
ACGAGCT6900.060.917079
CACGAGC20700.060.562598
CAACGCA7950.058.3408749
ACAACGC8100.058.1509788
Produced by FastQC (version 0.11.5)