FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_15_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_15_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2599013
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT158810.6110396523603383No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT135230.5203129033983285No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT116070.44659261034862074No Hit
GAAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAAC99220.38176030670104383No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT92510.35594281367580693No Hit
CACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA90380.34774739487643963No Hit
CACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA88090.33893635776350484No Hit
AAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACA84920.3267394199259488No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT78090.30046021316553634No Hit
ATGTTATATTGGTCTACTGTTGTTTGCTTCAAAACTGTTTTGGCCTCATT75880.29195698520938523No Hit
ATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGT74550.28683965797785543No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC73030.2809912839989642No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT68680.2642541610988479No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT63180.2430922815699652No Hit
CTCTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT61690.23735933602486792No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG58660.22570106421168343No Hit
GGATGAAGTGGGCCATGTGTTCTAACTTTCCCTTGGCTCTACCCAGATCT52440.20176890227174699No Hit
AGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACAA51350.19757500251056845No Hit
GTATAGTGCTTTGTCCAATGACATTTCACAAGGAATTAAGAGACAAAGAA50210.19318872202640003No Hit
CATCTTGCTTTATCAAGGAAAACCAGTTAAAGTAGTGAAAAGGAAAAGGT49540.19061082033833612No Hit
ACCCAGATCTTGGAGATTTCAAAACTATTGAAGAACTTGAAAAGCTGAAA47150.18141502177942165No Hit
AATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTG44510.17125731960555796No Hit
GAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAAC43350.1667940868321936No Hit
AACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATT42140.16213847333583942No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT41940.16136895044388003No Hit
GTAGTAACAAGAGGATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT41880.16113809357629225No Hit
GTTAAAACAACTGCTAAGGGACAATGAGGCCAAAACAGTTTTGAAGCAAA41450.1594836193585796No Hit
GCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTC41030.15786762128546492No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT39570.15225010417416152No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT38580.1484409658589626No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT38220.14705582465343575No Hit
TCATTGGACAAAGCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTT38100.14659411091826013No Hit
GCTAAGGGACAATGAGGCCAAAACAGTTTTGAAGCAAACAACAGTAGACC38020.1462863017614764No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC37910.14586306417089873No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG37180.143054305615247No Hit
TCTTAATTCCTTGTGAAATGTCATTGGACAAAGCACTATACCTTTTCCTT36920.14205392585569984No Hit
GAATTAAGAGACAAAGAATGACAGTTGAGTCTATGGGGTGGGCCTTGAGC36880.14190002127730797No Hit
ACTTTAACTGGTTTTCCTTGATAAAGCAAGATGTTTGCCTTTTCCCCTGG36680.14113049838534858No Hit
ATTCAATACATCAAGGATTGAAAAGAATCCTTCACTAAGGATGAAGTGGG36290.1396299287460278No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT35730.1374752646485416No Hit
CTTAATTCCTTGTGAAATGTCATTGGACAAAGCACTATACCTTTTCCTTT35660.1372059316363558No Hit
GTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT35070.13493583910507567No Hit
GCTCTACCCAGATCTTGGAGATTTCAAAACTATTGAAGAACTTGAAAAGC33270.1280101330774413No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT32780.12612480199214088No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA31880.12266194897832369No Hit
GACCAATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAA31540.12135376006199278No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT31470.12108442704980699No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA31280.12035338030244559No Hit
GAATTAATGGATAAATTTATATTAGCTCAAGGCCCACCCCATAGACTCAA30540.11750614560219591No Hit
GGTTTCAGCTTTTCAAGTTCTTCAATAGTTTTGAAATCTCCAAGATCTGG29890.11500519620332794No Hit
AGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACAA29300.11273510367204782No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT29140.11211948535848032No Hit
GTTCTAACTTTCCCTTGGCTCTACCCAGATCTTGGAGATTTCAAAACTAT28650.11023415427317984No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA28420.10934920294742659No Hit
GTCCAATGACATTTCACAAGGAATTAAGAGACAAAGAATGACAGTTGAGT28380.1091952983690347No Hit
CAATTGCGTTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCCCA26980.1038086381253191No Hit
ACATTAGCCAAAATTGAATTGTTAAAACAACTGCTAAGGGACAATGAGGC26760.1029621629441638No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT26590.10230806848599834No Hit
CTCTTAATTCCTTGTGAAATGTCATTGGACAAAGCACTATACCTTTTCCT26450.10176940246162677No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA26000.1000379759547182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC13100.0125.0840451
GTAACAA66450.0123.815114
GCACGCA13300.0122.648492
CACGCAC13300.0122.643773
TAACAAG70700.0116.679795
GAAACAC31500.0112.760854
AAACACG32800.0108.2916645
TAGTAAC85500.097.089142
AGTAACA89450.092.879293
GTAGAAA41200.090.806571
GCTGCCC3500.087.00871145
AACACGT9100.084.423246
ACGTGCA19000.083.56452
GAAGCGG11500.083.222521
AGCGAAG353.438576E-582.8558652
AACACGA34350.081.443816
TAGAAAC46650.080.191692
AACAACG9200.079.5681847
TACGCGG2200.079.09883145
CAGCGTT1650.079.0790255
Produced by FastQC (version 0.11.5)