FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_13_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_13_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908248
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA72860.38181619999077687No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG64080.335805408940557No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA60600.31756878560857915No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC39800.20856827833698766No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT38360.2010220893720313No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG32860.17219983985310086No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG32230.16889838218093245No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA29440.15427764106132955No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA28350.14856559524757787No Hit
TCTCTCTTCTGTCCTTCCAAGCCTACTAAGCACGTGGCATCCTCCCAGAT27330.14322037806406715No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC25160.13184869052659823No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG24770.12980493101525586No Hit
CTGCATATCTGCTCCTTTGTATAAAGGATAAACAAAAACTTTAAAGTACT24510.12844242467436098No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC23100.1210534479795079No Hit
CCTTTATACAAAGGAGCAGATATGCAGAATGAATAAGTCTAGAAGATCTA22710.11900968846816556No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG22680.1188524761980623No Hit
GTAGTAACAAGAGCATAGAAAACCAAGCAAATGAGAAAAACAATGTAATT20990.1099961849822455No Hit
ATACAAAGGAGCAGATATGCAGAATGAATAAGTCTAGAAGATCTAGTGTG20260.1061706864097329No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG20160.10564664550938871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA56700.073.507861
ACGATCC5200.069.724686145
TAGTAAC60000.069.0934452
TTACGCC3950.066.08841145
TACGCGG1250.063.812035145
ACAACGC16350.061.1640788
AACAACG16350.061.1640787
TGACGCC6200.059.64834145
ACGAGCG1102.5465852E-1159.2904749
GCCGACC3600.056.39952145
AGTAACA74600.055.8611263
GTAACAA75350.054.627534
CAACGCA23000.051.9865769
TAACAAG79250.051.5734635
AAGAGCG12150.048.907199
CAAGAGC84200.046.5607838
ACACGAG17350.045.1086047
AACACGA17550.044.5922056
ACAAGAG91450.044.3751077
CACGAGC17550.044.1816338
Produced by FastQC (version 0.11.5)