FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_11_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_11_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547525
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC97610.6307490993683462No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT87810.5674221741167348No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA65490.4231918708906156No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA53220.3439039757031389No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC47720.30836335438845897No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT39070.2524676499571897No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT36270.2343742427424436No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG36030.2328233792668939No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG29630.19146701991890278No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT26900.1738259478845253No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA26260.16969031194972617No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA25320.16361609667048999No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA24570.15876964830939727No Hit
GTTTATATGGTCTCCAGAGACAACGTTTCATGCTCCATCTGTCTATAAGG23820.15392319994830456No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA23310.1506276150627615No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT22340.1443595418490816No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATG21770.14067624109465113No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT21440.13854380381577033No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC20260.13091872506098448No Hit
GTAAGAAGTACAGCAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA19610.12671846981470414No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT19470.1258137994539668No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT19120.12355212355212356No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA18890.12206587938805513No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT18030.11650861860066881No Hit
CTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTGATGCTAGCTA17490.11301917578068206No Hit
ATTGTATTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCCCACC17030.11004668745254519No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTGAT16950.10952973296069532No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT16560.10700957981292708No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG16390.10591105151774609No Hit
GAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGTTAAAAC16180.10455404597664011No Hit
GCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG15980.1032616597470154No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG15810.10216313145183437No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGCG100.0070889653145.01552145
AGCACGC20150.0123.057321
GCACGCA20800.0119.1963652
CACGCAC21150.0117.2200553
GTAACAA42800.0110.088454
GAAACAC12950.0104.675254
AAACACG13250.0101.20785
TAGTAAC47850.098.9307862
AGTAACA51200.092.31323
CGCACTT27350.091.701595
ACGCACT27600.091.136544
GTAGTAA53350.089.015021
GCCGACC1900.087.77255145
TAACAAG54700.086.533445
ACGTGCA7050.083.290632
AACACGT3750.083.101936
ACGATCC2950.073.73671145
AAGAGCC9200.070.109379
ACAAGAG68550.068.719477
GAACGCC651.3975296E-766.930244145
Produced by FastQC (version 0.11.5)