FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_117_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_117_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3303315
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT66690.20188810331439783No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC59600.1804248156775845No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT40760.12339119944661651No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC40680.12314901848597545No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA40640.12302792800565493No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA40070.12130238866108743No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG38970.11797240045227295No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC38490.11651931468842662No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT38180.11558086346594254No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT38110.11536895512538163No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA37500.11352232530049358No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC37080.11225087525712805No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG34910.10568171669973951No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA34360.10401672259533228No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT33790.10229118325076478No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA33730.10210954753028396No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT33600.10171600346924226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA46650.096.4989854
GAAACAC15650.091.2503744
AAACACG16300.086.274755
TGACGCC5500.085.6865145
GAGCAGA5850.083.0437551
GCTGCCC6150.078.98806145
AACACGT4300.077.531846
ACACGAG14350.074.748167
ACACGTG4550.073.271857
AACACGA14750.072.721096
TAGGGTG300.001937347672.4885945
CAACGCA4700.072.476519
TAACAAG62450.072.19845
TCTCGGG8950.072.098915145
GTCGCCC4250.069.945145
TAGTAAC65850.068.9203342
CACGAGC15800.067.429668
GAGCCCC1100.065.9127145
GCCGACC5200.065.532425145
CGCACCC954.5656634E-1061.055973145
Produced by FastQC (version 0.11.5)