FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_112_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_112_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112315
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA45710.21639764902488504No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC45080.21341513931397543No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA42880.2030000260377832No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG39580.18737735612349482No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT37330.17672553572738914No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT35070.16602637390730077No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG34810.16479549688375078No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA34510.16337525416427No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT30710.14538551305084704No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC30090.14245034476392016No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG30050.14226097906798937No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC26410.1250287007382895No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC26330.12464996934642798No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG25580.12109936254772607No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG25500.12072063115586454No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC25210.11934772986036647No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG24110.11414017322227035No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC23570.11158373632720497No Hit
ATATGTTATCATCAATCCTTTTACTTAAAAGATAAAGTTCTTCCGTGACT23190.10978476221586268No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT23060.10916932370408769No Hit
GGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGTCAAGAATAAA22470.10637617968910885No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAATTCACTC22320.10566605832936848No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA22040.1043404984578531No Hit
CATATGACCAGAGTGGAAGACTTGTTGCTAAACTTGTTGCTACTGATGAT21970.10400910848997427No Hit
GTATTGTCCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAA21880.10358303567413005No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA21700.10273089004244158No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA21190.10031647741932428No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA4200.0100.090382
AGCACGC4800.089.1186141
CACGCAC4850.086.676193
TGACGCC3650.085.4209145
GCCGACC2250.077.34233145
ACACGTG2550.073.8881457
GTAACAA46000.072.790924
CGAGGAT406.67593E-572.475815
CAACGCA5700.071.199249
AAACACG8150.071.14195
TCTCGGG5400.069.82294145
CGGAGCG1050.069.0245745
AGCGTTT2650.068.36538
TAACAAG49100.068.3427665
GAAACAC8750.067.920184
AGACGCC754.8530637E-967.674545145
GCTGCCC4450.066.80553145
ACAACGC6050.065.8792958
AACACGT2950.063.866396
GTCGCCC2500.063.807423145
Produced by FastQC (version 0.11.5)