FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_110_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_110_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401610
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA25710.10705318515495854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGA13600.070.334866
AAACACG21300.068.393595
TGACGCC4900.065.11103145
ACAACGC7850.064.62368
ACGAGCG808.11815E-963.4132239
AACACGT8050.062.1139076
ACACGAG15500.061.7170267
CACGAGC15800.060.0865028
TAGTAAC46300.059.4916232
CGACGAA7450.059.370613145
GTAGTAA48050.058.390631
AACAACG9050.055.2539377
CAACGCA11050.053.124469
GCCGACC3950.051.3995145
GTAACAA56050.047.8447274
AGTAACA58250.046.7881743
TAACAAG59200.045.665275
ACGAGCA11050.045.254179
GAAACAC33050.045.175584
TCTCGGG12650.044.709732145
Produced by FastQC (version 0.11.5)