FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_10_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_10_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177930
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT54500.1714952815197314No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA53350.16787657374454443No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA49850.1568631152983231No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT47590.14975156784447738No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC46530.14641606328647894No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT44810.14100373513576447No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG42720.13442712709216378No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA42520.13379778660952255No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC37690.11859921395373718No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG37010.11645945631275706No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT36550.11501197320268224No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT36030.11337568794781508No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC35210.1107953919689861No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC34650.10903323861759069No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGCTCTTCCGTGACC33870.10657881073528996No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA33680.1059809372767808No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT33490.10538306381827164No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG33210.10450198714257394No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT32870.10343210832208388No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT32570.10248809759812205No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGCGGGT32300.10163848794655642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA44350.0102.9561164
AAACACG11700.096.0191655
GAAACAC12050.091.424334
TAACAAG54050.083.944215
TGACGCC5900.082.33904145
GCACGCA1750.078.69892
AGCACGC1800.076.530891
GCCGACC4600.075.66003145
AACACGT3500.074.5357366
ACACGAG11550.074.0396357
TAGTAAC63150.073.576272
AACACGA11900.072.4652946
TACGCGG2900.070.00726145
TTCGGAA1150.069.35502145
GCTGCCC8000.067.06946145
ACGTGCA6150.066.003362
AGTAACA73400.063.2996643
GAGCAGA7150.062.869691
ACACGTG4250.061.3871967
GAAGCGG7800.060.4191251
Produced by FastQC (version 0.11.5)