FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_109_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_109_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146055
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC28600.085.406052
GTAGTAA29900.082.9202651
CGACGAA4800.071.00253145
GCACGCA9200.066.966232
AAACACG21750.066.638415
AGCACGC9250.066.616681
AACACGA15350.064.67496
AGTAACA40150.060.6538353
ACACGAG16750.060.135887
CACGAGC17450.056.8929948
GTAACAA45850.052.7923664
ACGAGCA7750.052.3636749
TAACAAG48250.050.465655
CGGAGCG1457.2759576E-1249.9788135
CGCACTT11950.047.302125
GAAGCGG13050.044.441151
AACACGT11100.043.7396556
TGACGCC6300.043.738136145
ACGCACT16050.043.3471154
GAAACAC34300.043.1022644
Produced by FastQC (version 0.11.5)