FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_105_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_105_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946228
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT86020.2919665416254275No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA78160.2652883619326135No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC64260.21810939275575414No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC56280.19102391260961474No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA54680.18559323989860932No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT52530.17829577344319583No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT49670.1685884459722737No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT48260.1638026656457002No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA48070.16315777326126832No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA46560.158032575890257No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT46300.1571500915747186No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT45620.1548420556725413No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA44660.15158365204593807No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG44100.14968291659708616No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC41960.14241939184611646No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATG39610.1344430913018273No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT39420.1337981989173954No Hit
ATCCAATGATGACCAATAACCCCATATACATCTTCGGAGCTTATGTGTAC39340.13352666528184512No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA39140.13284783119296945No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT39020.13244053073964404No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC38520.13074344551745484No Hit
ATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATA37200.12626314053087542No Hit
ATATAAATCCTTACTTTCTCTTCATAGATGTACCCATACAGGCAGCAATT36240.12300473690427217No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA34140.1158769789710776No Hit
CTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACA33720.11445142738443867No Hit
ATCTGGGACTCATAGCTGGAGAACTAAAAGGAACAGATCTATACTAAATA32210.10932623001342734No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT31280.10616965150015546No Hit
ATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCAT31280.10616965150015546No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT31220.10596600127349275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACAC21400.0102.288094
AGCACGC5350.098.917871
GCACGCA5500.096.203772
AAACACG23350.094.056255
AAGAGCC16950.093.640159
CACGCAC5900.092.138513
GTCGCCC3350.090.90729145
GTAACAA63000.085.943294
TGACGCC5100.081.0399145
AACACGA21600.079.5156866
ACACGAG22050.077.569527
AACACGT6550.076.342426
GCCGACC3750.075.40976145
GCTGCCC4950.074.70664145
ACAACGC5500.073.792648
CAACGCA5450.073.139829
ACACGTG6850.073.003927
TCTCGGG7500.072.509384145
TAACAAG75200.071.8075945
TAGTAAC77250.071.4032752
Produced by FastQC (version 0.11.5)