FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_102_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_102_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188003
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC38400.079.096792
CGACGAA6200.073.677376145
GTAGTAA41850.073.275821
AAACACG24250.072.9330445
AGCACGC6300.072.4962841
ACACGAG18350.069.517337
GTAACAA45250.066.479194
AACACGA19600.065.821916
ACGAGCG1150.063.0272759
GCACGCA7400.062.6937522
CACGAGC20250.060.8471688
AGTAACA50450.060.3467943
CGGAGCG851.308581E-859.6932685
ACGAGCC5300.058.8056379
TGACGCC6900.057.796143145
GCCGACC1950.055.7753145
AACACGT9100.055.7523546
TAACAAG57150.052.7622575
ACAACGC11000.051.3947038
GAAACAC35150.050.5239034
Produced by FastQC (version 0.11.5)