FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_100_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_100_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450364
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC77240.3152184736635047No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT77190.31501442234704724No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT56040.228700715485536No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT47720.19474657642701249No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC45930.1874415392978349No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA34410.1404281159860331No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC31940.13034798095303393No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC30780.12561399041122054No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT29920.12210430776815201No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG29390.1199413638137028No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC29080.11867624565166644No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT28930.11806409170229401No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC28360.11573790669467882No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG26760.10920826456803968No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA26730.10908583377816519No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC26730.10908583377816519No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA26560.1083920593022098No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG26530.1082696285123353No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA26300.10733099245663093No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA25750.10508642797559874No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA25630.10459670481610078No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA25510.10410698165660284No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC25470.10394374060343688No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA24980.10194403770215363No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT24840.10137269401607273No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT24800.10120945296290675No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC24630.10051567848695132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC5200.0114.3207241
GTAACAA46700.0113.2981644
GCACGCA5500.0106.753872
CACGCAC5600.0103.551013
AAACACG16850.0101.0845645
GAAACAC16950.0100.91584
TAACAAG55400.095.636685
GCCGACC2450.079.90342145
ACGTGCA7050.078.142222
TAGTAAC68950.077.4808652
TGACGCC3200.077.036514145
CGCACTT7850.075.7113655
AACACGA18800.075.170836
ACGCACT8150.074.7030944
ACACGAG19200.073.6092767
GAGCAGA5050.071.778281
GCTGCCC4950.071.77258145
TAGAAAC25950.070.671522
AACACGT4150.069.8529056
TCTCGGG5000.069.60476145
Produced by FastQC (version 0.11.5)