FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10898346
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA22050.083.0643162
TAACAAG127200.073.363215
TAGTAAC131800.072.064442
AAACACG49300.070.0461045
CACGCAC26750.069.552043
CGCACTT28550.062.6330225
ACGCACT33300.057.6105774
ACACGAG47750.055.328687
GTAACAA179650.052.4667664
AACACGT18000.046.6551936
GTAGTAA224000.043.673051
CGGAGCG6000.041.0242085
ACAACGC25350.040.832028
AGAAGCG16000.036.6503035
AACAACG28500.036.571987
ACACGTG23650.034.584237
AACAAGA292100.032.2696276
ACAAGAG300900.031.392017
CACGAGC82750.031.1404428
GCAGAGC109150.026.7952753