FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_POSITIVE_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_POSITIVE_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2599545
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT133270.5126666397388774No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT125510.482815261901602No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC102370.39379968417549993No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT75170.2891659886634007No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT68160.26219973110678985No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT55950.21522997293757176No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT48300.18580174607479386No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT45310.17429973322254472No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC44870.17260712932455488No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG44150.16983741385511697No Hit
GTACTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTGTGT43460.1671831031969056No Hit
GTATAATGGAGACCCATGGGCTGACAGTGATGCCCTAGCTTTTAGTGGAG41600.16002800490085764No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT35930.13821649557903404No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT35390.13613920897695558No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT33390.12844555489518356No Hit
GTTTTCATAACCTCTTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA32620.12548349807370135No Hit
GAAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGG32570.12529115672165705No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT30690.11805912188479137No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT30630.11782831226233821No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATACTAAGAACAC29960.1152509381449446No Hit
CCCCCAGACCAGATGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG29570.11375067559899905No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATTGTTCAAACA29040.11171185726732948No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC29030.11167338899692061No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT28600.11001925336933964No Hit
GTATTATTATCACTATATGTGTCAGCTTTATTATCATACTTACTATATTC28490.10959610239484217No Hit
CCATTATACTTGACATACAGTCCCATCCCCATCCTTTCAGTCTTAGACAT28490.10959610239484217No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT28350.10905754660911814No Hit
GAACTAGCAGAAAAATTACACTGTTGGTTTGGTGGGAAAGAATTTGACCT28040.10786503022644349No Hit
CACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA27730.10667251384376882No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGCAAACAAG27330.10513378302741443No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC27020.10394126664473975No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA26800.10309496469574483No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT26560.10217172620593218No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC26560.10217172620593218No Hit
AAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAATAATTGTACTAC26330.10128695598652841No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA100.0070842933145.04941
GCGTCAG100.0070842933145.04941
CCCGATA100.007087554145.027073
GCGAGGA100.007087554145.027074
ATGTGCG100.007092855144.99087
GGCCCCG100.007092855144.9908145
GACTATG100.007092855144.99087
GTAACAA58450.0129.891654
AGCACGC20100.0126.286781
CACGCAC20800.0122.017963
GCACGCA20850.0121.7253652
GAGCAGA3050.0121.2708051
GAAACAC20750.0120.564684
AAACACG22400.0111.360085
TAACAAG70900.0107.389585
TAGAAAC27850.096.858292
ACGCACT27250.095.797694
CGCACTT27350.095.447435
TAGTAAC83200.091.6006242
CAGCGTT900.088.627655
Produced by FastQC (version 0.11.5)